Purification and Kinetic Studies of the Citrate Cleavage Enzyme
نویسندگان
چکیده
منابع مشابه
The Citrate Cleavage Enzyme
This enzyme is widely distributed in animal tissues and has been partially purified from pigeon liver (1) and e.xtensively purified from chicken liver (2). Because of the complexity of the overall reaction, we attempted at first to fractionate the activity into more than one protein fraction. The results of these earlier studies led to the conclusion that one protein component or tightly bound ...
متن کاملThe Citrate Cleavage Enzyme
This enzyme is widely distributed in animal tissues and has been partially purified from pigeon liver (1) and e.xtensively purified from chicken liver (2). Because of the complexity of the overall reaction, we attempted at first to fractionate the activity into more than one protein fraction. The results of these earlier studies led to the conclusion that one protein component or tightly bound ...
متن کاملThe purification of citrate-condensing enzyme.
The citrate-condensing enzyme was isolated as a crystalline protein from pig heart by Ochoa et al. (1). These workers reported that pig heart contained considerable quantities of the condensing enzyme. We were surprised when we obtained much higher yields of this enzyme from pig heart tissue with a different extraction procedure (2). This observation led to a purification procedure that yields ...
متن کاملCitrate cleavage enzyme and fatty acid synthesis.
A series of experiments was performed to test the proposal of others that citrate cleavage enzyme (EC 4.1.3.8) regulates the rate of fatty acid synthesis in rat liver. The results show that, in recovery from fasting, fatty acid synthesis increased markedly without change in citrate cleavage enzyme activity. After the administration of alloxan, fatty acid synthesis decreased prior to any change ...
متن کاملCitrate Cleavage Enzyme and Fatty Acid Synthesis*
A series of experiments was performed to test the proposal of others that citrate cleavage enzyme (EC 4.1.3.8) regulates the rate of fatty acid synthesis in rat liver. The results show that, in recovery from fasting, fatty acid synthesis increased markedly without change in citrate cleavage enzyme activity. After the administration of alloxan, fatty acid synthesis decreased prior to any change ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)95802-3